Isolation of recombinant plasmids bearing cDNA to hen ovomucoid and lysozyme mRNAs.
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چکیده
منابع مشابه
Isolation of recombinant plasmids bearing cDNA to hen ovomucoid and lysozyme mRNAs.
A large library of hen oviduct cDNA-pCR1 recombinant plasmids has been established in Escherichia coli X1776. From this library, ovomucoid cDNA and lysozyme cDNA-bearing plasmids have been identified. One of these plasmids, pMu7, yielded the sequence of the 3'-untranslated region of ovomucoid mRNA.
متن کاملAn integrated process for purification of lysozyme, ovalbumin, and ovomucoid from hen egg white.
This article describes an integrated process for simultaneous purification of lysozyme, ovalbumin, and ovomucoid from hen egg white. The crude egg white extract was passed through a cation exchanger Streamline trade mark SP and the bound lysozyme was eluted with 5% ammonium carbonate, pH 9.0, containing 1 M NaCl after elution of avidin. This partially purified lysozyme was further purified 639-...
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Hen ovomucoid is characterized by a high degree of microheterogeneity of its carbohydrate moieties, as was recently demonstrated by hydrazinolysis in combination with high performance liquid chromatography (Paz Parente, J., Strecker, G., Leroy, Y., Montreuil, J., and Fournet, B. (1982) J. Chromatogr., in press). This approach resulted in 17 oligosaccharide-alditol fractions; the major one could...
متن کاملSynthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.
Sequential reverse transcriptase, DNA polymerase, and S1 nuclease reactions can be employed to synthesize double-stranded DNA representing messenger RNA. Using reverse transcriptase products made from partially purified lysozyme, ovomucoid, and ovalbumin messengers from hen oviduct, we have characterized the Escherichia coli DNA polymerase I reaction. We have optimized for a high yield of full ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)86841-2